Ribosite ®Ornithobacterium rhinotracheale ORT Real-time PCR

The qualitative testing with Ribosite® ORT kits is based on a duplex Real-Time PCR: In one reaction setting, the target genes for Ornithobacterium rhinotracheale as well as for the exogenous control (Internal Amplification Control (IAC))are amplified in parallel by respective primer pairs in the Polymerase Chain Reaction (PCR). Amplified target gene fragments are detected via fluorescently labeled probes during the PCR reaction in real-time (Real-Time PCR). The probes specific for detection of amplified Ornithobacterium rhinotracheale and the exogenous control target genes are labeled with fluorescent dyes FAM and HEX, respectively, and their emitted fluorescence is separately optically measured by the Real-Time PCR thermal cycler. By means of both individual analyses in one reaction vessel per sample and the Negative Control and Positive Control per run the Ornithobacterium rhinotracheale-specific status of a sample can be evaluated in the end. This way, results can be achieved within a few hours after sample receipt.